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recombinant murine klotho (R&D Systems)

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R&D Systems recombinant murine klotho
Recombinant Murine Klotho, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+kl/10__1161_slash_jaha__125__045175-59-13-23?v=R%26D+Systems
Average 94 stars, based on 50 article reviews

recombinant murine klotho - by Bioz Stars, 2026-07

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Klotho alleviated DQ-induced cardiomyocyte oxidative stress. (A, B) Cell viability was examined using Cell Counting Kit-8 (CCK-8) assay. (C) Klotho protein levels were examined using western blot analysis. (D) DQ-stimulated H9c2 cardiomyocytes were treated with recombinant Klotho protein, followed by viability assessment using CCK-8 assay. (E, F) Apoptosis was examined using flow cytometry. (G, H) ROS fluorescence signal, (I) MDA level, (J) GSH-ST, (K) GSH-PX, and (L) SOD activities in H9c2 cells were assessed using ELISA kits. (M, N) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. Data were presented as mean ± SD. * P <0.05, ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.

Journal: American Journal of Translational Research

Article Title: Klotho mitigates diquat-induced myocardial injury in rats by activating Nrf2/ARE-mediated suppression of oxidative stress

doi: 10.62347/SKBR3572

Figure Lengend Snippet: Klotho alleviated DQ-induced cardiomyocyte oxidative stress. (A, B) Cell viability was examined using Cell Counting Kit-8 (CCK-8) assay. (C) Klotho protein levels were examined using western blot analysis. (D) DQ-stimulated H9c2 cardiomyocytes were treated with recombinant Klotho protein, followed by viability assessment using CCK-8 assay. (E, F) Apoptosis was examined using flow cytometry. (G, H) ROS fluorescence signal, (I) MDA level, (J) GSH-ST, (K) GSH-PX, and (L) SOD activities in H9c2 cells were assessed using ELISA kits. (M, N) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. Data were presented as mean ± SD. * P <0.05, ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.

Article Snippet: To induce oxidative injury, cells were treated with 50 μM DQ (45422, Sigma-Aldrich, USA) for 24 h. For Klotho intervention, cells were treated with 1 μg/mL recombinant Klotho protein (1819-KL, R&D Systems, USA) [ 19 , 20 ].

Techniques: Cell Counting, CCK-8 Assay, Western Blot, Recombinant, Flow Cytometry, Fluorescence, Enzyme-linked Immunosorbent Assay, Control

Nrf2 inhibition abrogated the protective effects of Klotho on DQ-induced oxidative stress. DQ-stimulated H9c2 cells received recombinant Klotho protein and Nrf2 inhibitor ML385. (A, B) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. (C) Cell viability was examined using CCK-8 assay. (D, E) Apoptosis was examined using flow cytometry. (F, G) ROS fluorescence signal, (H) MDA level, (I) GSH-ST, (J) GSH-PX, and (K) SOD activities in H9c2 cells were assessed using ELISA kits. Data were presented as mean ± SD. ** P <0.01, compared to control group. ## P <0.01, compared to DQ group. && P <0.01, compared to DQ+Klotho group.

Journal: American Journal of Translational Research

Article Title: Klotho mitigates diquat-induced myocardial injury in rats by activating Nrf2/ARE-mediated suppression of oxidative stress

doi: 10.62347/SKBR3572

Figure Lengend Snippet: Nrf2 inhibition abrogated the protective effects of Klotho on DQ-induced oxidative stress. DQ-stimulated H9c2 cells received recombinant Klotho protein and Nrf2 inhibitor ML385. (A, B) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. (C) Cell viability was examined using CCK-8 assay. (D, E) Apoptosis was examined using flow cytometry. (F, G) ROS fluorescence signal, (H) MDA level, (I) GSH-ST, (J) GSH-PX, and (K) SOD activities in H9c2 cells were assessed using ELISA kits. Data were presented as mean ± SD. ** P <0.01, compared to control group. ## P <0.01, compared to DQ group. && P <0.01, compared to DQ+Klotho group.

Techniques: Inhibition, Recombinant, Western Blot, CCK-8 Assay, Flow Cytometry, Fluorescence, Enzyme-linked Immunosorbent Assay, Control

Klotho alleviated DQ-induced acute myocardial injury in rats through Nrf2/ARE activation. Rats were intragastrically administered with DQ to induce acute myocardial injury and treated with recombinant Klotho protein for 5 days. (A, B) Klotho protein expression in myocardial tissues was assessed using immunohistochemistry. (C) H&E staining of myocardial tissue of rats in each group (D, E) ROS fluorescence signal, (F) MDA level, (G) GSH-ST, (H) GSH-PX, and (I) SOD activities in H9c2 cells were assessed using ELISA kits. (J, K) Nrf2, HO-1, and NQO1 protein levels in myocardial tissues was assessed using immunohistochemistry. ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.

Journal: American Journal of Translational Research

Article Title: Klotho mitigates diquat-induced myocardial injury in rats by activating Nrf2/ARE-mediated suppression of oxidative stress

doi: 10.62347/SKBR3572

Figure Lengend Snippet: Klotho alleviated DQ-induced acute myocardial injury in rats through Nrf2/ARE activation. Rats were intragastrically administered with DQ to induce acute myocardial injury and treated with recombinant Klotho protein for 5 days. (A, B) Klotho protein expression in myocardial tissues was assessed using immunohistochemistry. (C) H&E staining of myocardial tissue of rats in each group (D, E) ROS fluorescence signal, (F) MDA level, (G) GSH-ST, (H) GSH-PX, and (I) SOD activities in H9c2 cells were assessed using ELISA kits. (J, K) Nrf2, HO-1, and NQO1 protein levels in myocardial tissues was assessed using immunohistochemistry. ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.

Techniques: Activation Assay, Recombinant, Expressing, Immunohistochemistry, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay, Control

TGF-β1 induces EMT and downregulates klotho expression in ARPE-19 cells. (A) TGF-β1-induced morphological changes in APRE-19 cells. Scale bar, 200 μ m. (B) Expression levels of EMT-associated markers in response to TGF-β1 stimulation were detected by western blotting. (C) Relative protein expression (normalized to GAPDH) in western blot. (D) Cell Counting Kit-8 assay was used to determine the proliferation of ARPE-19 cells treated with 10 ng/ml TGF-β1. (E) EdU assays were used to confirm proliferation of ARPE-19 cells treated with 10 ng/ml TGF-β1 for 24 h. Scale bar, 100 μ m. (F) Quantification of the proliferative ability of cells in EdU assays. (G) Representative migration of ARPE-19 cells treated with TGF-β1. (H) Quantification of the migratory ability of cells in Transwell migration assay. Scale bar, 400 μ m. (I) Wound healing of ARPE-19 cells treated with TGF-β1Scale bar, 200 μ m. (J) Quantification of the migratory ability of cells in scratch assay. (K) Relative mRNA expression (normalized to GAPDH) of klotho in cells treated with or without TGF-β1. (L) Relative expression of klotho in cells treated with or without TGF-β1 were detected by western blotting. (M) Quantification of the relative protein expression (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 vs. control group. EMT, epithelial-mesenchymal transition; SMA, smooth muscle actin; ZO, zona occludens; Cad, cadherin; OD, optical density.

Journal: International Journal of Molecular Medicine

Article Title: Klotho attenuates epithelial-mesenchymal transition of retinal pigment epithelial cells in subretinal fibrosis by suppressing the ERK1/2 and Wnt/β-catenin signaling pathways

doi: 10.3892/ijmm.2025.5486

Figure Lengend Snippet: TGF-β1 induces EMT and downregulates klotho expression in ARPE-19 cells. (A) TGF-β1-induced morphological changes in APRE-19 cells. Scale bar, 200 μ m. (B) Expression levels of EMT-associated markers in response to TGF-β1 stimulation were detected by western blotting. (C) Relative protein expression (normalized to GAPDH) in western blot. (D) Cell Counting Kit-8 assay was used to determine the proliferation of ARPE-19 cells treated with 10 ng/ml TGF-β1. (E) EdU assays were used to confirm proliferation of ARPE-19 cells treated with 10 ng/ml TGF-β1 for 24 h. Scale bar, 100 μ m. (F) Quantification of the proliferative ability of cells in EdU assays. (G) Representative migration of ARPE-19 cells treated with TGF-β1. (H) Quantification of the migratory ability of cells in Transwell migration assay. Scale bar, 400 μ m. (I) Wound healing of ARPE-19 cells treated with TGF-β1Scale bar, 200 μ m. (J) Quantification of the migratory ability of cells in scratch assay. (K) Relative mRNA expression (normalized to GAPDH) of klotho in cells treated with or without TGF-β1. (L) Relative expression of klotho in cells treated with or without TGF-β1 were detected by western blotting. (M) Quantification of the relative protein expression (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 vs. control group. EMT, epithelial-mesenchymal transition; SMA, smooth muscle actin; ZO, zona occludens; Cad, cadherin; OD, optical density.

Article Snippet: Recombinant mouse klotho protein (cat. no. 1819-KL, R&D Systems, Inc.) or PBS was administered intravitreally 3 days after photocoagulation.

Techniques: Expressing, Western Blot, Cell Counting, Migration, Transwell Migration Assay, Wound Healing Assay, Control

Klotho overexpression attenuates EMT in ARPE-19 cells induced by TGF-β1. ARPE-19 cells were infected with Lv-klotho or Lv-NC, followed by treatment with or without TGF-β1. (A) Protein levels of EMT-associated markers were analyzed using western blotting. (B) Relative ZO-1 protein expression (normalized to GAPDH) in western blot. (C) Quantification of the relative α-SMA protein expression (normalized to GAPDH) in western blot. (D) Quantification of the relative N-cad protein expression (normalized to GAPDH) in western blot. (E) Quantification of the relative collagen I protein expression (normalized to GAPDH) in western blot. (F) Cell Counting Kit-8 assays were used to determine the proliferation of ARPE-19 cells (G) EdU assays were used to assess the proliferation of ARPE-19 cells. Scale bar, 100 μ m. (H) Quantification of the proliferative ability of cells in EdU assays. (I) Representative Transwell cell migration assay. Scale bar, 200 μ m. (J) Quantitative analysis of the migratory ability of cells. (K) Wound healing assay was used to assess cell migration. Scale bar, 200 μ m. (L) Quantification of the migratory ability of cells in wound healing assay. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. Lv-NC, lentiviral vector negative control; EMT, epithelial-mesenchymal transition; SMA, smooth muscle actin; ZO, zona occludens; Cad, cadherin; OD, optical density.

Journal: International Journal of Molecular Medicine

Article Title: Klotho attenuates epithelial-mesenchymal transition of retinal pigment epithelial cells in subretinal fibrosis by suppressing the ERK1/2 and Wnt/β-catenin signaling pathways

doi: 10.3892/ijmm.2025.5486

Figure Lengend Snippet: Klotho overexpression attenuates EMT in ARPE-19 cells induced by TGF-β1. ARPE-19 cells were infected with Lv-klotho or Lv-NC, followed by treatment with or without TGF-β1. (A) Protein levels of EMT-associated markers were analyzed using western blotting. (B) Relative ZO-1 protein expression (normalized to GAPDH) in western blot. (C) Quantification of the relative α-SMA protein expression (normalized to GAPDH) in western blot. (D) Quantification of the relative N-cad protein expression (normalized to GAPDH) in western blot. (E) Quantification of the relative collagen I protein expression (normalized to GAPDH) in western blot. (F) Cell Counting Kit-8 assays were used to determine the proliferation of ARPE-19 cells (G) EdU assays were used to assess the proliferation of ARPE-19 cells. Scale bar, 100 μ m. (H) Quantification of the proliferative ability of cells in EdU assays. (I) Representative Transwell cell migration assay. Scale bar, 200 μ m. (J) Quantitative analysis of the migratory ability of cells. (K) Wound healing assay was used to assess cell migration. Scale bar, 200 μ m. (L) Quantification of the migratory ability of cells in wound healing assay. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. Lv-NC, lentiviral vector negative control; EMT, epithelial-mesenchymal transition; SMA, smooth muscle actin; ZO, zona occludens; Cad, cadherin; OD, optical density.

Article Snippet: Recombinant mouse klotho protein (cat. no. 1819-KL, R&D Systems, Inc.) or PBS was administered intravitreally 3 days after photocoagulation.

Techniques: Over Expression, Infection, Western Blot, Expressing, Cell Counting, Cell Migration Assay, Wound Healing Assay, Migration, Plasmid Preparation, Negative Control

Knockdown of klotho induces EMT in ARPE-19 cells. ARPE-19 cells were infected with lentiviral plasmids harboring sh-klotho or empty vector. (A) Klotho expression was measured using RT-qPCR following klotho overexpression. (B) Protein expression levels of klotho. (C) Quantification of the relative protein expression (normalized to GAPDH) in western blot. (D) Klotho expression was measured using RT-qPCR following knockdown. (E) Protein expression levels of EMT-related proteins were detected following klotho knockdown. (F) Quantification of the relative protein expression (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001 vs. sh-NC. sh, short hairpin; RT-q, reverse transcription-quantitative; NC, negative control; EMT, epithelial-mesenchymal transition; SMA, smooth muscle actin; ZO, zona occludens; Cad, cadherin; Lv, lentiviral vector.

Journal: International Journal of Molecular Medicine

Article Title: Klotho attenuates epithelial-mesenchymal transition of retinal pigment epithelial cells in subretinal fibrosis by suppressing the ERK1/2 and Wnt/β-catenin signaling pathways

doi: 10.3892/ijmm.2025.5486

Figure Lengend Snippet: Knockdown of klotho induces EMT in ARPE-19 cells. ARPE-19 cells were infected with lentiviral plasmids harboring sh-klotho or empty vector. (A) Klotho expression was measured using RT-qPCR following klotho overexpression. (B) Protein expression levels of klotho. (C) Quantification of the relative protein expression (normalized to GAPDH) in western blot. (D) Klotho expression was measured using RT-qPCR following knockdown. (E) Protein expression levels of EMT-related proteins were detected following klotho knockdown. (F) Quantification of the relative protein expression (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001 vs. sh-NC. sh, short hairpin; RT-q, reverse transcription-quantitative; NC, negative control; EMT, epithelial-mesenchymal transition; SMA, smooth muscle actin; ZO, zona occludens; Cad, cadherin; Lv, lentiviral vector.

Article Snippet: Recombinant mouse klotho protein (cat. no. 1819-KL, R&D Systems, Inc.) or PBS was administered intravitreally 3 days after photocoagulation.

Techniques: Knockdown, Infection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Over Expression, Western Blot, Reverse Transcription, Negative Control

Bioinformatics analysis following klotho overexpression. (A) Principal component analysis and (B) volcano plot of significant DEGs. (C) GO and (D) enrichment of genes and pathways. DEG, differentially expressed gene; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; Lv, lentiviral vector; NC, negative control; FDR, false discovery rate; FC, fold-change; PCA, principal components analysis.

Journal: International Journal of Molecular Medicine

Article Title: Klotho attenuates epithelial-mesenchymal transition of retinal pigment epithelial cells in subretinal fibrosis by suppressing the ERK1/2 and Wnt/β-catenin signaling pathways

doi: 10.3892/ijmm.2025.5486

Figure Lengend Snippet: Bioinformatics analysis following klotho overexpression. (A) Principal component analysis and (B) volcano plot of significant DEGs. (C) GO and (D) enrichment of genes and pathways. DEG, differentially expressed gene; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; Lv, lentiviral vector; NC, negative control; FDR, false discovery rate; FC, fold-change; PCA, principal components analysis.

Article Snippet: Recombinant mouse klotho protein (cat. no. 1819-KL, R&D Systems, Inc.) or PBS was administered intravitreally 3 days after photocoagulation.

Techniques: Over Expression, Plasmid Preparation, Negative Control

Klotho regulates EMT of ARPE-19 cells via the ERK1/2 and Wnt/β-catenin signaling pathway. Protein expression levels of (A) ERK signaling pathway were analyzed using western blotting. (B) Relative protein expression. (C) β-catenin pathway were analyzed using western blotting. (D) Quantification of the relative protein expression of β-catenin. (E) Quantification of the relative protein expression of c-Myc. (F) Quantification of the relative protein expression of CyclinD1 (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001. EMT, epithelial-mesenchymal transition; Lv, lentiviral vector; NC, negative control; p, phosphorylated.

Journal: International Journal of Molecular Medicine

Article Title: Klotho attenuates epithelial-mesenchymal transition of retinal pigment epithelial cells in subretinal fibrosis by suppressing the ERK1/2 and Wnt/β-catenin signaling pathways

doi: 10.3892/ijmm.2025.5486

Figure Lengend Snippet: Klotho regulates EMT of ARPE-19 cells via the ERK1/2 and Wnt/β-catenin signaling pathway. Protein expression levels of (A) ERK signaling pathway were analyzed using western blotting. (B) Relative protein expression. (C) β-catenin pathway were analyzed using western blotting. (D) Quantification of the relative protein expression of β-catenin. (E) Quantification of the relative protein expression of c-Myc. (F) Quantification of the relative protein expression of CyclinD1 (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001. EMT, epithelial-mesenchymal transition; Lv, lentiviral vector; NC, negative control; p, phosphorylated.

Article Snippet: Recombinant mouse klotho protein (cat. no. 1819-KL, R&D Systems, Inc.) or PBS was administered intravitreally 3 days after photocoagulation.

Techniques: Expressing, Western Blot, Plasmid Preparation, Negative Control

Klotho alleviates progression of subretinal fibrosis in a mouse model of choroidal neovascularization. (A) Representative confocal images of RPE-choroid-sclera flat-mounts stained for collagen I in mice with subretinal fibrosis treated with 10 or 20 nM klotho. Scale bar, 100 μ m. (B) Quantitative analysis of collagen I-positive lesion area in the mice. (C) Representative Masson staining of collagen fibers (black arrow). Magnification, ×200, Scale bar, 200 μ m. (D) Semiquantitative analyses of the Masson staining showed that subretinal deposition of collagen was alleviated following klotho treatment. (E) Representative confocal images of eye sections stained for RPE65 (green) and α-SMA (red), showing the presence of RPE65 + α-SMA + cells (white arrows) in the fibrotic lesion. Cell nuclei were stained with DAPI (blue). Scale bar, 10 μ m. (F) Western blotting was used to determine protein expression levels of epithelial-mesenchymal transition-related markers. (G) Relative ZO-1 protein expression (normalized to GAPDH) in western blot. (H) Quantification of relative α-SMA protein expression (normalized to GAPDH) in western blot. (I) Quantification of the relative N-cad protein expression (normalized to GAPDH) in western blot. (J) Quantification of the relative collagen I protein expression (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. RPE, retinal pigment epithelium; SMA, smooth muscle actin; ZO, zona occludens; cad, cadherin.

Journal: International Journal of Molecular Medicine

Article Title: Klotho attenuates epithelial-mesenchymal transition of retinal pigment epithelial cells in subretinal fibrosis by suppressing the ERK1/2 and Wnt/β-catenin signaling pathways

doi: 10.3892/ijmm.2025.5486

Figure Lengend Snippet: Klotho alleviates progression of subretinal fibrosis in a mouse model of choroidal neovascularization. (A) Representative confocal images of RPE-choroid-sclera flat-mounts stained for collagen I in mice with subretinal fibrosis treated with 10 or 20 nM klotho. Scale bar, 100 μ m. (B) Quantitative analysis of collagen I-positive lesion area in the mice. (C) Representative Masson staining of collagen fibers (black arrow). Magnification, ×200, Scale bar, 200 μ m. (D) Semiquantitative analyses of the Masson staining showed that subretinal deposition of collagen was alleviated following klotho treatment. (E) Representative confocal images of eye sections stained for RPE65 (green) and α-SMA (red), showing the presence of RPE65 + α-SMA + cells (white arrows) in the fibrotic lesion. Cell nuclei were stained with DAPI (blue). Scale bar, 10 μ m. (F) Western blotting was used to determine protein expression levels of epithelial-mesenchymal transition-related markers. (G) Relative ZO-1 protein expression (normalized to GAPDH) in western blot. (H) Quantification of relative α-SMA protein expression (normalized to GAPDH) in western blot. (I) Quantification of the relative N-cad protein expression (normalized to GAPDH) in western blot. (J) Quantification of the relative collagen I protein expression (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. RPE, retinal pigment epithelium; SMA, smooth muscle actin; ZO, zona occludens; cad, cadherin.

Article Snippet: Recombinant mouse klotho protein (cat. no. 1819-KL, R&D Systems, Inc.) or PBS was administered intravitreally 3 days after photocoagulation.

Techniques: Staining, Western Blot, Expressing

Klotho alleviates progression of subretinal fibrosis in vivo via ERK1/2 and Wnt/β-catenin signaling pathway. (A) Protein expression levels of ERK pathway were analyzed using western blotting. (B) Quantification of the relative protein expression (normalized to GAPDH) in western blot. (C) β-catenin pathway were analyzed using western blotting. (D) Relative β-catenin protein expression (normalized to GAPDH) in western blot. (E) Quantification of the relative c-Myc protein expression (normalized to GAPDH) in western blot. (F) Quantification of the relative cyclinD1 protein expression (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. p, phosphorylated.

Journal: International Journal of Molecular Medicine

Article Title: Klotho attenuates epithelial-mesenchymal transition of retinal pigment epithelial cells in subretinal fibrosis by suppressing the ERK1/2 and Wnt/β-catenin signaling pathways

doi: 10.3892/ijmm.2025.5486

Figure Lengend Snippet: Klotho alleviates progression of subretinal fibrosis in vivo via ERK1/2 and Wnt/β-catenin signaling pathway. (A) Protein expression levels of ERK pathway were analyzed using western blotting. (B) Quantification of the relative protein expression (normalized to GAPDH) in western blot. (C) β-catenin pathway were analyzed using western blotting. (D) Relative β-catenin protein expression (normalized to GAPDH) in western blot. (E) Quantification of the relative c-Myc protein expression (normalized to GAPDH) in western blot. (F) Quantification of the relative cyclinD1 protein expression (normalized to GAPDH) in western blot. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. p, phosphorylated.

Article Snippet: Recombinant mouse klotho protein (cat. no. 1819-KL, R&D Systems, Inc.) or PBS was administered intravitreally 3 days after photocoagulation.

Techniques: In Vivo, Expressing, Western Blot

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